Cat#:RP-1668M;Product Name:Recombinant Mouse BCA 1 Protein;Synonym:C-X-C motif chemokine 13, B lymphocyte chemoattractant, CXC chemokine BLC, Small-inducible cytokine B13, Cxcl13, Blc, Scyb13.;Description:CXCL13 Mouse Recombinant (22-109) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10kDa. The BCA-1 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:ILEAHYTNLK CRCSGVISTV VGLNIIDRIQ VTPPGNGCPK TEVVIWTKMK KVICVNPRAK WLQRLLRHVQ SKSLSSTPQA PVSKRRAA.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured by its ability to chemoattract human CXCR5-transfected mouse BaF3 cells, is less than 2µg/ml.;Formulation:The BCA1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized CXCL13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
CXCL13 Mouse Recombinant (22-109) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 88 amino acids and having a molecular mass of 10kDa. The BCA-1 is purified by proprietary chromatographic techniques.
The ED50, as measured by its ability to chemoattract human CXCR5-transfected mouse BaF3 cells, is less than 2µg/ml.
Formulation:
The BCA1 protein was lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized CXCL13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized BCA1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BCA1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.