Cat#: | RP-1372M |
Product Name: | Recombinant Mouse ITK Kinase Protein (aa 351-619, His & GST Tag) |
Synonym: | Emt,Tcsk,Tsk |
Description: | A DNA sequence encoding the mouse ITK / EMT (Q03526) protein kinase domain (Arg 351-Leu 619) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
Source: | Baculovirus-Insect Cells |
Predicted N Terminal: | Met |
Molecular Characterization: | The secreted recombinant mouse ITK /GST chimera consists of 506 amino acids and has a calculated molecular mass of 58.4 kDa. The recombinant protein migrates as an approximately 58 kDa band in SDS-PAGE under reducing conditions. |
Purity: | Greater than 95 % as determined by SDS-PAGE |
Endotoxin: | < 1.0 EU per ug of the protein as determined by the LAL method |
Bioactivity: | Please contact us for detailed information |
Formulation: | Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 7.5, 10% gly, 0.5mM GSH |
Stability: | Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃ |
Reconstitution: | Please see related COA for specific instructions. |
Storage: | Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃). |