Human XRCC5 (+floxed/-); RAD54B (+/-/-) HCT116 Cell Line Online Inquiry

• Product Type:
• Knockout Stable Cell Line

• Cat#:
• ABKSC17102

• Product Name:
• Human XRCC5 (+floxed/-); RAD54B (+/-/-) HCT116 Cell Line

• Size:
• 1 vial

• Description:
• Human XRCC5 (+floxed/-); RAD54B (+/-/-) HCT116 Cell line is Knockout of single XRCC5 allele and flanking of exon 3 in remaining allele with LoxP sites; double knockout of RAD54B in triploid parental cells

• Host Species:
• Human

• Biosafety Level:
• 1

• Growth Properties:
• Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:10-1:20.

• Recommended Medium:
• Growth Medium: RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.; Freeze Medium: Complete growth media 45%; USDA approved FBS 50%; DMSO 5%; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents

• Quality Control:
• Genotype: XRCC5 (+floxed/-); RAD54B (+/-/-)

• Storage:
• The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase