Cat#:ABKSC11339;Product Name:Human PDIA3 Knockout Stable Cell Line;Size:1 vial;Synonym:ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HEL-S-269, HEL-S-93n, HsT17083, P58, PI-PLC;Description:Human PDIA3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.;Source:HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat;Host Species:Human;Storage:The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase;
Human PDIA3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.
Source:
HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat
Host Species:
Human
Biosafety Level:
1
Growth Properties:
Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Recommended Medium:
Growth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish
Quality Control:
Genotype: PDIA3 HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat knockout. >95% viability before freezing. All cells were tested and found to be free of bacterial, viruses, mycoplasma and other toxins.
Storage:
The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase