Human KRAS (G12D/+) LIM1215 Cell Line Online Inquiry

• Product Type:
• Knockin Stable Cell Line

• Cat#:
• ABKSC8069

• Product Name:
• Human KRAS (G12D/+) LIM1215 Cell Line

• Size:
• 1 vial

• Description:
• Human KRAS (G12D/+) LIM1215 Cell line is Heterozygous knockin of KRAS activating mutation (G12D)

• Host Species:
• Human

• Biosafety Level:
• 2

• Growth Properties:
• Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:4-1:8. Media change every 3-4 days. Cells may experience a lag period of approximat

• Recommended Medium:
• Growth Medium: RPMI 1640 including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS, 1ug/ml Insulin, 1 ug/ml hydrocortisone.; Freeze Medium: Complete growth media 45%; USDA approved FBS 50%; DMSO 5%; Revival: Rapidly thaw cells

• Quality Control:
• Genotype: KRAS (G12D/+)

• Storage:
• The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase