Cat#:ABKSC8072;Product Name:Human KRAS (G12D/+/+/+) EBC-1 Cell Line;Size:1 vial;Description:Human KRAS (G12D/+/+/+) EBC-1 Cell line is Heterozygous knockin of KRAS activating mutation (G12D);Host Species:Human;Storage:The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase;
Human KRAS (G12D/+/+/+) EBC-1 Cell line is Heterozygous knockin of KRAS activating mutation (G12D)
Host Species:
Human
Biosafety Level:
1
Growth Properties:
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 70-80% confluency, approximately 1:4-1:6.
Recommended Medium:
Growth Medium: EMEM including 2mM L-glutamine and 25mM sodium bicarbonate, supplemented with 10% FBS.; Freeze Medium: Complete growth media 45%; USDA approved FBS 50%; DMSO 5%; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into
Quality Control:
Genotype: KRAS (G12D/+/+/+)
Storage:
The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase