Human GPC3 Knockout Stable Cell Line Online Inquiry

• Product Type:
• Knockout Stable Cell Line

• Cat#:
• ABKSC6278

• Product Name:
• Human GPC3 Knockout Stable Cell Line

• Synonym:
• DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1

• Size:
• 1 vial

• Description:
• Human GPC3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

• Source:
• HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat

• Host Species:
• Human

• Biosafety Level:
• 1

• Growth Properties:
• Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.

• Recommended Medium:
• Growth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish

• Quality Control:
• Genotype: GPC3 HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat knockout. >95% viability before freezing. All cells were tested and found to be free of bacterial, viruses, mycoplasma and other toxins.

• Storage:
• The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase