Cat#:ABKSC5531;Product Name:Human FGFR1 Knockout HAP1 Cell Line;Size:1 vial;Synonym:bFGF-R-1,BFGFR,CD331,CEK,FGFBR,FGFR-1,FLG,FLT-2,FLT2,HBGFR,HH2,HRTFDS,KAL2,N-SAM,OGD;Source:HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat;Host Species:Human;Storage:The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase;
Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish.
Recommended Medium:
Growth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Quality Control:
Genotype: FGFR1 HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat knockout. >95% viability before freezing. All cells were tested and found to be free of bacterial, viruses, mycoplasma and other toxins.
Storage:
The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase