Human BRCA1 (185delAG/+) MCF10A Cell Line Online Inquiry

• Product Type:
• Knockin Stable Cell Line

• Cat#:
• ABKSC1545

• Product Name:
• Human BRCA1 (185delAG/+) MCF10A Cell Line

• Size:
• 1 vial

• Description:
• Human BRCA1 (185delAG/+) MCF10A Cell line is Heterozygous knockin of 2-bp deletion in BRCA1 resulting in a premature termination codon at position 39

• Host Species:
• Human

• Biosafety Level:
• 1

• Growth Properties:
• Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 3-5 days. Split at 80-90% confluency, approximately 1:6-1:10.

• Recommended Medium:
• Growth Medium: DMEM/F-12 including 2.5 mM L-glutamine and 15 mM HEPES, supplemented with 5% horse serum, 10 µg/mL insulin, 20 ng/mL hEGF, 0.5 µg/mL hydrocortisone, 0.1 µg/mL cholera toxin.; Freeze Medium: Complete growth media 45%; USDA approved FBS

• Quality Control:
• Genotype: BRCA1 (185delAG/+)

• Storage:
• The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase