Product Type: | Knockout Stable Cell Line |
Cat#: | ABKSC4989 |
Product Name: | Human EXT1 Knockout Stable Cell Line |
Synonym: | EXT, LGCR, LGS, TRPS2, TTV |
Size: | 1 vial |
Description: | Human EXT1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Source: | HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat |
Host Species: | Human |
Biosafety Level: | 1 |
Growth Properties: | Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20. |
Recommended Medium: | Growth Medium: IMDM, 10% FCS; Freeze Medium: IMDM, 20% FCS, 10% DMSO; Revival: Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 ml of pre-warmed media in a 10cm dish |
Quality Control: | Genotype: EXT1 HEK293T / HeLa / A549 / HCT116 / HEPG2 / Jurkat knockout. >95% viability before freezing. All cells were tested and found to be free of bacterial, viruses, mycoplasma and other toxins. |
Storage: | The cell line is shipped using dry ice. Upon arrival, it should be stored in liquid nitrogen vapor phase |