Cat#:RP-6423H;Product Name:Recombinant Human VEGFB Protein;Synonym:Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB.;Description:Recombinant human VEGFB Protein produced in E.coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids (22-207) and having a molecular mass of 21.6 kDa (molecular weight on SDS-PAGE will appear higher). The human VEGFB protein is fused to a 20 amino acid His Tag at N-terminus and purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA.;Purity:Greater than 90.0% as determined by SDS-PAGE.;Bioactivity:Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml corresponding to a specific activity of less than 1,000,000units/mg.;Formulation:The recombinant human VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol.;Stability:Recombinant human vegfb Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store recombinant human VEGFB protein at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;References:Poesen K, Lambrechts D, etc. "Novel role for vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration". J. Neurosci. 28 (42): 10451–9. ;