Cat#:RP-6375H;Product Name:Recombinant Human Uricase Protein;Synonym:Urate Oxidase, Uricase, Urate Oxygen, Oxidoreductase, UOX, UO, EC 1.7.3.;Description:Urate Oxidase Recombinant produced in E.coli is a tetrameric, non-glycosylated polypeptide chain containing 302 amino acids, having a molecular formula of C1523H2383N417O462S7 and a molecular mass of 34,247 Dalton. The cDNA coding for urate-oxidase was cloned from a strain of Aspergillus flavus . The monomer protein has no intra- or inter-disulfide bridges.;Source:E.coli;AA Sequence:msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tm;Purity:Greater than 96.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The specific activity was found to be 10U/mg. One Unit oxidizes one micromole of uric acid per minute at 25°C, at pH 8.5.;Formulation:Each 1.5mg Uricase contains 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6 mg Na2HPO4*12H20 and 0.33 mg NaH2PO4*2H20.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:We highly recommend reconstituting the lyophilized Uricase in 50mM borate buffer containing 0.001%Triton X-100 and 1.0mM EDTA, pH 8.5 for activity assay.;Storage:Lyophilized Urate Oxidase although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Uricase should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;