Cat#:RP-262AC;Product Name:Recombinant Human TNFSF11 / RANKL / CD254 Protein(His Tag);Synonym:RANKL,CD254,TRANCE,OPGL,ODF;Background:This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq, Jul 2008];Description:Human TNFSF11 with His Tag is produced in HEK293 cells. It contains AA Gly 64 - Asp 245.;Source:HEK293;Predicted N Terminal:His;Molecular Characterization:Recombinant Human TNFSF11 / RANKL / CD254 Protein has a calculated MW of 23.1 kDa. The recombinant protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.;Purity:Greater than 95% as determined by SDS-PAGE.;Endotoxin:< 1.0 EU per μg of recombinant human TNFSF11 / RANKL / CD254 protein by the LAL method.;Formulation:Recombinant human TNFSF11 / RANKL / CD254 protein was lyophilized from 0.22 μm filtered solution in PBS, pH7.4.;Stability:Recombinant human TNFSF11 / RANKL / CD254 protein was stable up to 1 year from date of receipt at -70℃;Storage:Store recombinant human TNFSF11 / RANKL / CD254 protein under sterile conditions at -20℃ to -80℃. It is recommended that the recombinant protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;References:Proinsulin C-peptide modulates the expression of ERK1/2, type I collagen and RANKL in human osteoblast-like cells (Saos-2). Russo C, et al. Mol Cell Endocrinol, 2017 Feb 15. PMID 28007656;