Cat#:RP-6110H;Product Name:Recombinant Human TIGAR Protein, TAT;Synonym:Fructose-2,6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5.;Description:TIGAR Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 283 amino acids (including the 270 residues of full-length TIGAR and a 13-residue C-terminal TAT peptide) and having a molecular mass of 31.7kDa. The TIGAR is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PN;Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The Specific Activity was measured by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 µg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.;Formulation:TIGAR was Lyophilized from a 0.2µm filtered concentrated solution in 20mM PBS, pH7.0, 350mM NaCl and 5% Trehalose.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized TIGAR in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized TIGAR stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution TIGAR should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;