Cat#:RP-6064H;Product Name:Recombinant Human TGF b 1 Protein;Synonym:Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1.;Description:Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa. The TGF-b 1 is purified by proprietary chromatographic techniques.;Source:Human Platelets.;Purity:Greater than 98.0% as determined by SDS-PAGE.;Bioactivity:Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 200,000-10,000,000IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF- must;Formulation:TGF-Beta1 protein was lyophilized from a solution containing 30% acetonitrile and 0.1% trifluoroacetic acid. ;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute lyophilized TGF-beta 1 in 0.5% BSA in 0.1N acetic acid, which can then be further diluted to the desired aliquot with 30% acetonitrile and 0.1% trifluoroacetic acid.;Storage:Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C. DO NOT RECONSTITE WITH NEUTRAL BUFFERS. DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS. PREVENT FREEZE THAW CYCLES.;
Human Transforming Growth Factor-beta 1 purified from Human Platelets having a molecular mass of 25kDa. The TGF-b 1 is purified by proprietary chromatographic techniques.
Source:
Human Platelets.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Bioactivity:
Stimulates the growth of NRK-1 cells in soft agar at concentrations ranging from 0.1 to 5ng/ml corresponding to a specific activity of 200,000-10,000,000IU/mg. Effective concentration ranges must be experimentally determined. Purified EGF and/or TGF- must
Formulation:
TGF-Beta1 protein was lyophilized from a solution containing 30% acetonitrile and 0.1% trifluoroacetic acid.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute lyophilized TGF-beta 1 in 0.5% BSA in 0.1N acetic acid, which can then be further diluted to the desired aliquot with 30% acetonitrile and 0.1% trifluoroacetic acid.
Storage:
Lyophilized TGF-beta 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGF-beta 1 should be stored at 4°C between 2-7 days and for future use below -18°C. DO NOT RECONSTITE WITH NEUTRAL BUFFERS. DO NOT USE GLASS IMPLEMENTS OR EXTENSIVE MANIPULATIONS. PREVENT FREEZE THAW CYCLES.