Cat#:RP-5980H;Product Name:Recombinant Human T4 DNA;Synonym:DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.;Description:T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.;Source:Escherichia Colilambda lysogen NM 989.;Bioactivity:One Weiss unit is equivalent to circa 67 cohesive-end ligation units. • T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM. • Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much e;Formulation:50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 µg/ml BSA and DNA (0.1 to 1 µm in 5´ termini). Optimal ligation occurs at 16C.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;