Cat#:RP-5621H;Product Name:Recombinant Human Stem Cell Factor / SCF Protein;Synonym:Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.;Background:Gene ID: 4254.KITLG, the full name is KIT ligand, also known as SCF, SF, MGF, SLF, DFNA69,DCUA, FPHH, KL-1, FPH2, Kitl, SHEP7. This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration.;Description:Stem Cell Factor / SCF Protein human produced in E.coli is a single, non-glycosylated polypeptide chain containing 165aa and having a MW of 18.4 kDa. The human SCF protein is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.;Molecular Characterization:Approximately 18.4 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.;Purity:Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 of recombinant human SCF proteinas determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.;Formulation:The human SCF protein was lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3.;Stability:Recombinant SCF Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized scf protein in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Host Species:Human ;Storage:Lyophilized KIT ligand / SCF protein although stable at room temperature for 3 weeks, should be stored at -20 or -70℃. Upon reconstitution SCF human recombinant protein should be stored at 4°C between 2-7 days and for future use below -20℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;References:Expression of the stem cell factor in fibroblasts, endothelial cells, and macrophages in periapical tissues in human chronic periapical diseases. Shen SQ, et al. Genet Mol Res, 2017 Mar 8. ;