Cat#:RP-5832H;Product Name:Recombinant Human SPA-Cys Long;Synonym:Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SPA.;Description:SPA-Cys long Recombinant produced in E.coli is a single non-glycosylated polypeptide chain with a Cys on C-terminus. ?SPA-Cys is comprised of 5 IgG-binding domains E-D-A-B-C aligned in series containing 423 amino acids and having a molecular mass of 46.7kDa containing little or no carbohydrate.?;Source:E.coli;AA Sequence:AQHDEAQQNA FYQVLNMPNL NADQRNGFIQ SLKDDPSQSA NVLGEAQKLN DSQAPKADAQ QNNFNKDQQS AFYEILNMPN LNEAQRNGFI QSLKDDPSQS TNVLGEAKKL NESQAPKADN NFNKEQQNAF YEILNMPNLN EEQRNGFIQS LKDDPSQSAN LLSEAKKLNE SQAPKADNKF NKEQQNAFYE ILHLPNLNEE QRNGFIQSLK DDPSQSANLL AEAKKLNDAQ AP;Purity:Greater than 97.0% as determined by analysis by SDS-PAGE.;Formulation:SPA protein was lyophilized with no additives.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized SPA in sterile 18MΩ-cm H2O not less than 0.1mg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized SPA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SPA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).? Please prevent freeze-thaw cycles.;