Cat#:RP-5735H;Product Name:Recombinant Human SIRT3 Protein;Synonym:Sirtuin 3, SIR2-like protein 3, Sir2-like 3, NAD-dependent deacetylase sirtuin-3, mitochondrial.;Description:SIRT3 produced in E.coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (118-399a.a.) and having a molecular mass of 33.5kDa. SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MSDKGKLSLQ DVAELIRARA CQRVVVMVGA GISTPSGIPD FRSPGSGLYS NLQQYDLPYP EAIFELPFFF HNPKPFFTLA KELYPGNYKP NVTHYFLRLL HDKGLLLRLY TQNIDGLERV SGIPASKLVE AHGTFASATC TVCQRPFPGE DIRADVMADR VPRCPVCTGV VKPDIVFFGE PLPQRFLLHV VDFPMADLLL ILGTSLEVEP FA;Purity:Greater than 95% as determined by SDS-PAGE.;Formulation:The SIRT3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 0.1M NaCl, 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;
SIRT3 produced in E.coli is a single, non-glycosylated polypeptide chain containing 303 amino acids (118-399a.a.) and having a molecular mass of 33.5kDa. SIRT3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
The SIRT3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 1mM DTT, 0.1M NaCl, 10% glycerol.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.