Cat#:RP-5720H;Product Name:Recombinant Human SHH Protein;Synonym:SHH, HHG-1, HHG1, Sonic hedgehog protein, TPT, HLP3, HPE3, SMMCI, TPTPS, MCOPCB5.;Description:Sonic HedgeHog Recombinant Human produced in E.coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.1kDa.? The Cys at position 2 has been substituted with 2 Ile’s.?;Source:E.coli;AA Sequence:MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGGCFP;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is 1.2-1.8ug/ml corresponding to a specific activity of 800U/mg.;Formulation:SHH is lyophilized from 10mM Na3PO4, pH 7.5.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized SHH in sterile 10mM Na3PO4 pH 7.5 not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Human Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Sonic HedgeHog should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;