Cat#:RP-5544H;Product Name:Recombinant Human RSPO3 Protein;Synonym:R-spondin-3, Protein with TSP type-1 repeat, hPWTSR, Roof plate-specific spondin-3, hRspo3, Thrombospondin type-1 domain-containing protein 2, RSPO3, PWTSR, THSD2, THSD2, CRISTIN1.;Description:Recombinant Human R-Spondin-3 produced in HEK293 cells is a polypeptide chain starting at amino acid at position 22 to amino acid at position 200, fused to an FC-6xHis-tag at C-terminus, containing a total of 498 amino acids. RSPO3 is a truncated protein that lacks amino acid V at position 180 to amino acid H at position 251 and purified by proprietary chromatographic techniques.;Source:HEK293 cells.;AA Sequence:QNASRGRRQR RMHPNVSQGC QGGCATCSDY NGCLSCKPRL FFALERIGMK QIGVCLSSCP  SGYYGTRYPD INKCTKCKAD CDTCFNKNFC TKCKSGFYLH LGKCLDNCPE GLEANNHTME  CVSIVHCEVS EWNPWSPCTK KGKTCGFKRG TETRVREIIQ HPSAKGNLCP PTNETRKCTV  DDIEGRMDEP KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP;Purity:Greater than 95% as determined by SDS PAGE.;Formulation:RSPO3 was lyophilized from a 0.2µm filtered solution in PBS, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to quick spin followed by reconstitution of RSPO3 in PBS to a concentration no less than 100 µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized RSPO3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution RSPO3 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;