Cat#:RP-5333H;Product Name:Recombinant Human pykF Protein;Synonym:Pyruvate kinase I, PK-1, pykF, b1676, JW1666.;Description:pykF E.coli Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 494 amino acids (1-470 a.a) and having a molecular mass of 53.3kDa. pykF is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli.;AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMKKTKI VCTIGPKTES EEMLAKMLDA GMNVMRLNFS HGDYAEHGQR IQNLRNVMSK TGKTAAILLD TKGPEIRTMK LEGGNDVSLK AGQTFTFTTD KSVIGNSEMV AVTYEGFTTD LSVGNTVLVD DGLIGMEVTA IEGNKVICKV LNNGDLGENK GVNLPGVSIA LPALAEKDKQ DLIFGCEQGV DFVAASFIRK RSDVIEIREH LK;Purity:Greater than 95% as determined by SDS-PAGE.;Formulation:pykF protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;