Cat#:RP-5304H;Product Name:Recombinant Human PTH (1-84) Protein;Synonym:Parathyrin, PTH, Parathormone.;Description:Parathyroid Hormone 1-84 (full length) Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids, having an MW of ~10kDa. The PTH is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ.;Purity:Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 9000 Units/mg.;Formulation:Each 1.3mg of lyophilized protein contains 50µg Mannitol and 20mM PB, pH 7.0.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Parathormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Parathyroid Hormone 1-84 (full length) Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids, having an MW of ~10kDa. The PTH is purified by proprietary chromatographic techniques.
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 9000 Units/mg.
Formulation:
Each 1.3mg of lyophilized protein contains 50µg Mannitol and 20mM PB, pH 7.0.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized Parathormone in sterile 18MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized Parathyrin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PTH should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.