Cat#:RP-5172H;Product Name:Recombinant Human PPM1A Protein;Synonym:Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201.;Description:Protein Phosphatase 1A Alpha Isoform Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 418 amino acids and having a molecular mass of 46.6KDa (containing a 36 aa His tag, T7 gene 10 leader, XpressTM Epitope). The protein coding region of PPM1A (amino acids 1-382) was cloned into an E. coli expression vector (BamHI/Hind3 site). PP2Ca was overexpressed in E. coli as a soluble His-tag fusion protein, and it was purified by conventional column chromatographic techniques.;Source:E.coli;AA Sequence:MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWILMGAF LDKPKMEKHN AQGQGNGLRY GLSSMQGWRV EMEDAHTAVI GLPSGLESWS FFAVYDGHAG SQVAKYCCEH LLDHITNNQD FKGSAGAPSV ENVKNGIRTG FLEIDEHMRV MSEKKHGADR SGSTAVGVLI SPQHTYFINC GDSRGLLCRN RKVHFFTQDH KPSNPLEKER IQNAGGSVMI QRVNGSLAVS RA;Purity:Greater than 95.0% as determined by SDS-PAGE.;Formulation:PPM1A protein solution (1mg/ml) contains 10mM Tris-HCl, pH7.5, 50mM NaCl, 2mM DTT, 1mM MnCl2 and 20% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;