Cat#:RP-5105H;Product Name:Recombinant Human PLGF 1 Protein, Sf9;Synonym:PIGF, PGF, PLGF-1, PlGF-2.;Description:Placenta Growth Factor-1 Protein produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 131 amino acids and having a total molecular mass of approximately 34 kDa. The PLGF-1 is purified by proprietary chromatographic techniques.;Source:Insect Cells.;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Pro-Ala-Val.;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:The ED50 as determined by its ability to bind to immobilized rh-sFlt-1 in a functional ELISA. PlGF-1 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.5-10ng/ml, corresponding to a Specific Activity of 1x105-2x106un;Formulation:Lyophilized from a concentrated (1mg/ml) solution containing 50mM acetic acid.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Placenta Growth Factor 1 in sterile 0.1M acetic acid not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Placenta Growth Factor 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;