Cat#:RP-5071H;Product Name:Recombinant Human PIXY321 Protein;Synonym:PIXY321, GMCSF/ IL3.;Description:GMCSF/IL3 fusion protein Protein produced in E.coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kDa. PIXY321 is purified by proprietary chromatographic techniques.;Source:E.coli;Purity:Greater than 95.0% as determined by1. Analysis by RP-HPLC. 2. Analysis by SDS-PAGE.;Bioactivity:The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.;Formulation:The protein was lyophilized after extensive dialysis against 10mM PBS, pH= 7.4±0.1 and 0.1% BSA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized PIXY321 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PIXY321should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;
GMCSF/IL3 fusion protein Protein produced in E.coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kDa. PIXY321 is purified by proprietary chromatographic techniques.
Source:
E.coli
Purity:
Greater than 95.0% as determined by1. Analysis by RP-HPLC. 2. Analysis by SDS-PAGE.
Bioactivity:
The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.2ng/ml, corresponding to a Specific Activity of 5,000,000IU/mg.
Formulation:
The protein was lyophilized after extensive dialysis against 10mM PBS, pH= 7.4±0.1 and 0.1% BSA.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized PIXY321 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PIXY321should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.