Cat#:RP-4964H;Product Name:Recombinant Human PDGF CC Protein;Synonym:Platelet Derived Growth Factor C, Spinal Cord-Derived Growth Factor, FALLOTEIN, PDGF-C, VEGF-E, SCDGF, Secretory Growth Factor-Like Protein, Platelet-Derived Growth Factor C, PDGFC.;Description:PDGF-CC Protein (235-345) produced in E.coli is a disulfide-linked homodimer containing 2x118 amino acids and having a total molecular mass of 26.8kDa. The PDGF-CC is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminal and purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MHHHHHHVVD LNLLTEEVRL YSCTPRNFSV SIREELKRTD TIFWPGCLLV KRCGGNCACC LHNCNECQCV PSKVTKKYHE VLQLRPKTGV RGLHKSLTDV ALEHHEECDC VCRGSTGG.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 350ng/ml.;Formulation:Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized PDGF-CC in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized PDGF-CC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-CC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
PDGF-CC Protein (235-345) produced in E.coli is a disulfide-linked homodimer containing 2x118 amino acids and having a total molecular mass of 26.8kDa. The PDGF-CC is fused to a 7 amino acid His tag [M-HHHHHH] at N-terminal and purified by proprietary chromatographic techniques.
The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 350ng/ml.
Formulation:
Lyophilized from a 0.2µm filtered solution in Acetonitrile and TFA.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized PDGF-CC in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized PDGF-CC although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-CC should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.