Cat#:RP-4959H;Product Name:Recombinant Human PDGF AB Protein;Synonym:Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AB.;Description:Platelet-derived Growth Factor AB Protein is a heterodimeric, non-glycosylated, polypeptide chain containing 234 amino acids (and an N-terminal Met) consisting of 14.3kDa alpha-chain and 12.1 beta-chain having a total molecular mass of 26.4kDa.? PDGF-AB is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT. Beta chain: SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KP;Purity:Greater than 95.0% as determined by SDS-PAGE analysis.;Bioactivity:The ED50 as determined by the dose-dependent proliferation of mouse 3T3 indicator cells, is 1.4-2.1 ng/ml. This corresponds to a specific activity of 7.1x 105 units/mg.;Formulation:Lyophilized from 10mM AcOH (Acetic Acid).;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-AB in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Platelet-derived Growth Factor AB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;