Cat#:RP-087AC;Product Name:Recombinant Human PCSK9 (R194A) Protein;Synonym:PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9;Description:Human PCSK9 (R194A) with His Tag is produced in HEK293 cells. It contains AA Gln 31 - Gln 692.;Source:HEK293;Predicted N Terminal:Gln 31;Molecular Characterization:Recombinant Human PCSK9 (R194A) Protein R194APCSK9(Gln 31 - Gln 692)AAI166619.1Poly-hisHuman PCSK9 (R194A), His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 71.8 kDa. The predicted N-terminus is Gln 31. DTT-reduced Protein migrates as 20 kDa and 65 kDa in SDS-PAGE due to glycosylation and proteolytic digestion.The R194A mutation results in lower affinity of PCSK9 for LDLR.;Purity:Greater than 95% as determined by SDS-PAGE.;Endotoxin:< 1.0 EU per μg of recombinant human PCSK9 (R194A) protein by the LAL method.;Formulation:Recombinant human PCSK9 (R194A) protein was lyophilized from 0.22 μm filtered solution in PBS, pH7.4.;Stability:Recombinant human PCSK9 (R194A) protein was stable up to 1 year from date of receipt at -70℃;Storage:Store recombinant human PCSK9 (R194A) protein under sterile conditions at -20℃ to -80℃. It is recommended that the recombinant protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;