Cat#:RP-4797H;Product Name:Recombinant Human NRN1 Protein;Synonym:Neuritin 1, NRN1, NRN, dJ380B8.2, Neuritin.;Description:Recombinant Human NRN1 produced in E.coli cells is a non-glycosylated, homodimeric protein containing 2x88 amino acid chains and having a molecular mass of 19.4kDa. The NRN1 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:AGKCDAVFKG FSDCLLKLGD SMANYPQGLD DKTNIKTVCT YWEDFHSCTV TALTDCQEGA KDMWDKLRKE SKNLNIQGSL FELCGSGN.;Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 25ng/ml, corresponding to a specific activity of > 4.0 × 104 IU/mg.;Formulation:The NRN1 was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized NRN1 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized NRN1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NRN1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;