Cat#:RP-4309H;Product Name:Recombinant Human LGALS3 Protein;Synonym:Galectin-3, GAL3, MAC2, CBP35, GALB, GALIG, LGALS2, LGALS3, Galactose-specific lectin 3, Mac-2 antigen, IgE-binding protein, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Laminin-binding protein, Lectin L-29, L-31, Galactoside-binding protein, G;Description:LGALS3 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 249 amino acids and having a molecular mass of 26kDa. The LGALS3 is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SK;Purity:Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC.;Bioactivity:The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 µg/ml.;Formulation:The Galectin-3 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 3mM DTT.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized LGALS3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
LGALS3 Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 249 amino acids and having a molecular mass of 26kDa. The LGALS3 is purified by proprietary chromatographic techniques.
Greater than 97.0% as determined by analysis by SDS-PAGE and HPLC.
Bioactivity:
The ED50 as determined by its ability to agglutinate human red blood cells is less than 10 µg/ml.
Formulation:
The Galectin-3 protein was lyophilized from a 0.2µm filtered concentrated solution in PBS, pH 7.4 and 3mM DTT.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized LGALS3 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized Galectin-3 Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Galectin-3 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.