Cat#:RP-4274H;Product Name:Recombinant Human LBP Protein;Synonym:Lipopolysaccharide-binding protein, LBP, MGC22233.;Description:The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag has no protease site and can not be split off.;Source:Chinese Hamster Ovarian Cells (CHO).;Formulation:Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Every 10µg of recombinant human LBP should be reconstituted using 40µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.;Storage:Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.;
The Lipopolysaccharide Binding Protein is produced from human LBP transfected CHO-cells in serum free medium. Before transfection the complete human LBP-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant Human LBP was purified by his-tag with metal affinity purification with Talon and controlled by SDS page. Showing a 58kDa band on SDS-PAGE. Attention: His-tag has no protease site and can not be split off.
Source:
Chinese Hamster Ovarian Cells (CHO).
Formulation:
Recombinant Human LBP was lyophilized from a protein solution (0.25 mg/ml) containing phosphate-buffered saline, pH 7.2.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
Every 10µg of recombinant human LBP should be reconstituted using 40µl of sterile H2O. The solution can be than diluted with phosphate-buffered saline or other buffers.
Storage:
Lyophilized LBP Human Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution LBP should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.