Cat#:RP-4212H;Product Name:Recombinant Human KLK1 Protein;Synonym:KLK1, KLK-1, HK1, HK-1, KLKR, KLK6, Tissue Kallikrein, hKLK1, EC 3.4.21.35, Kidney/pancreas/salivary gland kallikrein, Kallikrein-1.;Description:KLK1 Protein produced in Yeast is a single, glycosylated, polypeptide chain containing 238 amino acids and having a molecular mass of 28-32 kDa. KLK1 Protein is purified by proprietary chromatographic techniques.;Source:Pichia Pastoris.;Purity:Greater than 98.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolyze 1 µmole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37°C Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).;Formulation:Lyophilized from a solution containing 1xPBS.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized KLK1 in sterile 18M-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.;Storage:Lyophilized KLK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
KLK1 Protein produced in Yeast is a single, glycosylated, polypeptide chain containing 238 amino acids and having a molecular mass of 28-32 kDa. KLK1 Protein is purified by proprietary chromatographic techniques.
Source:
Pichia Pastoris.
Purity:
Greater than 98.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolyze 1 µmole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37°C Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).
Formulation:
Lyophilized from a solution containing 1xPBS.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized KLK1 in sterile 18M-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.
Storage:
Lyophilized KLK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution KLK1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.