Cat#:RP-1702H;Product Name:Recombinant Human ITGAX & ITGB2 Heterodimer Protein;Synonym:CD11C, SLEB6, LAD, CD18, MF17, MFI7, LCAMB, LFA-1, MAC-1;Description:A DNA sequence encoding the extracellular domain (Met 1-Pro 1107) of human ITGAX (P20702) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asn 700) of human ITGB2 (P05107) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.;Source:Human Cells;Predicted N Terminal:Phe 20 & Gln 21;Molecular Characterization:The recombinant heterodimer of human ITGAX/ITGB2 comprises 1879 (1130+718) amino acids and has a calculated molecular mass of 205 (125+80) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGAX/ITGB2 heterodimer is approximately 95 &13;Purity:Greater than 90 % as determined by SDS-PAGE;Endotoxin:< 1.0 EU per ug of the protein as determined by the LAL method;Bioactivity:Please contact us for detailed information;Formulation:Lyophilized from sterile PBS, pH 7.4;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Please see related COA for specific instructions.;Storage:Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).;
A DNA sequence encoding the extracellular domain (Met 1-Pro 1107) of human ITGAX (P20702) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asn 700) of human ITGB2 (P05107) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
Source:
Human Cells
Predicted N Terminal:
Phe 20 & Gln 21
Molecular Characterization:
The recombinant heterodimer of human ITGAX/ITGB2 comprises 1879 (1130+718) amino acids and has a calculated molecular mass of 205 (125+80) kDa. As a result of glycosylation, the apparent molecular mass of rh ITGAX/ITGB2 heterodimer is approximately 95 &13
Purity:
Greater than 90 % as determined by SDS-PAGE
Endotoxin:
< 1.0 EU per ug of the protein as determined by the LAL method
Bioactivity:
Please contact us for detailed information
Formulation:
Lyophilized from sterile PBS, pH 7.4
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
Please see related COA for specific instructions.
Storage:
Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).