Cat#:RP-4148H;Product Name:Recombinant Human Internalin-A Protein;Synonym:Internalin-A;Description:Internalin Listeria monocytogenes Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 374 amino acids and having a molecular mass of 42.7kDa. The Internalin-A is fused with an 8 amino acid His tag at C-terminus.;Source:E.coli;AA Sequence:MANENNFLEI MSETKEIKIV NIPDNNLKKV LNKSLNKSEN SDLTVKDLES IEYLYGIAEN ISNIEGLEYC KNLKILSLQN NDNSKKENFN TITDLSPLKY LKNLVVLDLR NNKISDLSPL ENLTNLESLR LSGNNISNIS PLNKLESLTT LTLSYNEITD ISTISNLKNL THLALYNNKI EDISSLKENT KLQNLSLGFN KIKDISVLSN LKNLYDLSLE ENNIKSIKSL SN;Purity:Greater than 95.0% as determined by SDS-PAGE and HPLC.;Formulation:Internalin-A is lyophilized from a 0.2µm filtered concentrated solution in 20mM PB pH7.4 and 300mM NaCl.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Important note: If the concentration of NaCl is less than 150mM, the protein solution bec;Storage:Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Internalin Listeria monocytogenes Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 374 amino acids and having a molecular mass of 42.7kDa. The Internalin-A is fused with an 8 amino acid His tag at C-terminus.
Greater than 95.0% as determined by SDS-PAGE and HPLC.
Formulation:
Internalin-A is lyophilized from a 0.2µm filtered concentrated solution in 20mM PB pH7.4 and 300mM NaCl.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized Internalin-A in sterile 2XPBS not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Important note: If the concentration of NaCl is less than 150mM, the protein solution bec
Storage:
Lyophilized Internalin-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Internalin-A should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.