Cat#:RP-4128H;Product Name:Recombinant Human IL5 Protein, Sf9;Synonym:EDF, BCDFII, TRF, T-cell replacing factor, Eosinophil differentiation factor, B cell differentiation factor I, IL-5.;Description:Interleukin-5 Protein produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 124 amino acids (20-134a.a.) and having a molecular mass of 14.2kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa). IL5 is expressed with a 6 amino acid His-tag at C-Terminus and purified by proprietary chromatographic techniques.;Source:Sf9, Baculovirus cells.;AA Sequence:ADPIPTEIPT SALVKETLAL LSTHRTLLIA NETLRIPVPV HKNHQLCTEE IFQGIGTLES QTVQGGTVER LFKNLSLIKK YIDGQKKKCG EERRRVNQFL DYLQEFLGVM NTEWIIESHH HHHH.;Purity:Greater than 90.0% as determined by SDS-PAGE.;Bioactivity:Measured in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 for this effect is less or equal to 1.5 ng/ml.;Formulation:IL5 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;