Cat#:RP-4043H;Product Name:Recombinant Human IL2 Protein;Synonym:T-cell growth factor (TCGF), Aldesleukin, Lymphokine, IL-2.;Description:Recombinant human Interleukin-2 / IL2 Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa. The recombinant human IL2 protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:MAPTSSSTKK TQLQLEHLLL DLQMILNGIN NYKNPKLTRM LTFKFYMPKK ATELKHLQCL EEELKPLEEV LNLAQSKNFH LRPRDLISNI NVIVLELKGS ETTFMCEYAD ETATIVEFLN RWITFCQSII STLT.;Purity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.;Formulation:The protein (1mg/ml) was lyophilized after extensive dialysis against 20mM PBS pH=7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Interleukin 2 in 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human Interleukin-2 / IL2 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IL2 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.;
Recombinant human Interleukin-2 / IL2 Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 15kDa. The recombinant human IL2 protein is purified by our unique chromatographic techniques.
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 10,000,000 IU/mg.
Formulation:
The protein (1mg/ml) was lyophilized after extensive dialysis against 20mM PBS pH=7.4.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized Interleukin 2 in 20mM AcOH not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized recombinant human Interleukin-2 / IL2 protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human IL2 protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.