Cat#:RP-0853H;Product Name:Recombinant Human IL12A & IL27B Heterodimer Protein;Synonym:CLMF,IL-12A,NFSK,NKSF1,P35;Description:A DNA sequence encoding the human IL27B (NP_005746.2)(Met1-Lys229) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human IL12A (NP_000873.2)(Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human IL27B&IL12A heterodimer was purified.;Source:Human Cells;Predicted N Terminal:Arg 21 & Arg 23;Molecular Characterization:The recombinant heterodimer of human IL27B&IL12A comprises 425 (217+208) amino acids and has a calculated molecular mass of 48.3(24.3+24) KDa.;Purity:Greater than 90 % as determined by SDS-PAGE.;Endotoxin:<1.0 EU per ug protein as determined by the LAL method.;Bioactivity:Please contact us for detailed information;Formulation:Lyophilized from sterile PBS, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Please see related COA for specific instructions.;Storage:Avoid repeated freeze-thaw cycles. In lyophilized state for 1 year (4-8℃); Upon reconstitution under sterile conditions for 2 weeks (4-8℃) or 3 months (-20℃ to -70℃).;