Cat#:RP-3971H;Product Name:Recombinant Human IFN a 2a Protein;Synonym:Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN-a 2a.;Description:Interferon Alpha Human 2a Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 165 amino acids and having a molecular mass of 19241 Dalton. The Interferon-a 2a gene was obtained from human leukocytes. The IFN-A 2a is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:The sequence of the first five N-terminal amino acids was determined and was found to be Cys-Asp-Leu-Pro-Gln, conforming to the sequence of native human IFN-a. N-terminal methionine has been completely removed enzymatically.;Purity:Greater than 97.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The specific activity as determined in a viral resistance assay using bovine kidney MDBK cells was found to be 270,000,000 IU/mg.;Formulation:Lyophilized without additives.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Interferon-alpha 2a in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Interferon alpha 2a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IFN-alpha 2a should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;