Cat#:RP-3866H;Product Name:Recombinant Human HPRT1 Protein, Active;Synonym:Hypoxanthine Phosphoribosyltransferase 1, EC 2.4.2.8, HGPRTase, HGPRT, HPRT , Hypoxanthine-Guanine Phosphoribosyltransferase 1, Hypoxanthine-Guanine Phosphoribosyltransferase, Testicular Tissue Protein Li 89, Lesch-Nyhan Syndrome, HPRT1.;Description:HPRT1 Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 238 amino acids (1-218 a.a) and having a molecular mass of 26.7kDa. HPRT1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MATRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TG;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:Specific activity is > 15 units/mg and is defined as the amount of enzyme that catalyze the formation of 1 umole of guanosine 5’-monophosphate (GMP) per minute from guanine and phosphoribosyl pyrophosphate at pH 7.5 at 37C.;Formulation:HPRT1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) and 20% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;