Cat#:RP-1374HAT;Product Name:Recombinant Human His-PP2C alpha Protein;Synonym:Protein phosphatase 1A (formerly 2C) magnesium-dependent alpha isoform;Description:Protein phosphatase 2C(PP2C alpha) is a Mn2+- or Mg2+- dependent protein serine/threonine phosphatase that is essential for regulating cellular stress response in eukaryotes. The protein coding region of PP2C alpha (amino acids 1-382) was cloned into an E. coli expression vector. PP2C alpha was overproduced in E. coli as a soluble His tag fusion protein, and it was purified using the column chromatographic techniques.;Source:E.coli;AA Sequence:1-382aa;Molecular Characterization:46.6 kDa;Purity:Greater than 95% by SDS-PAGE;Endotoxin:< 1.0 EU per ug of protein as determined by LAL method.;Bioactivity:Specific activity is > 1,400 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmoles of p-nitrophenyl phosphate (pNPP) per minute at pH 7.5 at 37C.;Formulation:Liquid Solution;Stability:Recombinant Human His-PP2C alpha Protein was stable up to 1 year from date of receipt at -70℃;Concentration:1mg/mL;Storage Buffer:Recombinant Human His-PP2C alpha protein was supplied in 10 mM Tris-HCl, pH7.5, 50 mM NaCl, 2 mM DTT, 1 mM MnCl2, 20%glycerol.;Storage:Recombinant Human His-PP2C alpha Protein can be stored at +4℃for 1-2 weeks. It is recommended that the recombinant protein be aliquoted for optimal storage at -20 or -70℃. Please avoid repeated freeze-thaw cycles.;Usage:For Lab Research Use Only;