Cat#:RP-3515H;Product Name:Recombinant Human GPT Protein, Active;Synonym:Alanine aminotransferase 1, ALT1, EC 2.6.1.2, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1.;Description:Alanine Aminotransferase Protein produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT. The ALT is purified by proprietary chromatographic techniques.;Source:E.coli;Purity:Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The specific activity was found to be 1,020 U/mg.;Formulation:The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:AAT1 although stable at 10°C for 5 days, should be stored below -18°C. Please prevent freeze-thaw cycles.;
Alanine Aminotransferase Protein produced in E.coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT. The ALT is purified by proprietary chromatographic techniques.
Source:
E.coli
Purity:
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The specific activity was found to be 1,020 U/mg.
Formulation:
The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
AAT1 although stable at 10°C for 5 days, should be stored below -18°C. Please prevent freeze-thaw cycles.