Cat#:RP-3512H;Product Name:Recombinant Human GPNMB Protein, HEK;Synonym:Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN, GPNMB, HGFIN, NMB.;Description:GPNMB Protein produced in HEK cells is a single, glycosylated, polypeptide chain (a.a 22-486) containing a total of 477 amino acids, having a molecular mass of 53.7kDa (calculated), though it migrates at approximately 97kDa on SDS PAGE, the GPNMB is fused to a 2 a.a C-terminal linker and a 10 a.a His tag at C-Terminus. The Human GPNMB is purified by proprietary chromatographic techniques.;Source:HEK 293.;AA Sequence:AKRFHDVLGN ERPSAYMREH NQLNGWSSDE NDWNEKLYPV WKRGDMRWKN SWKGGRVQAV LTSDSPALVG SNITFAVNLI FPRCQKEDAN GNIVYEKNCR NEAGLSADPY VYNWTAWSED SDGENGTGQS HHNVFPDGKP FPHHPGWRRW NFIYVFHTLG QYFQKLGRCS VRVSVNTANV TLGPQLMEVT VYRRHGRAYV PIAQVKDVYV VTDQIPVFVT MFQKNDRNSS DE;Purity:Greater than 95.0% as determined by SDS-PAGE.;Formulation:Filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer and 0.075M NaCl, pH 7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. GPNMB is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.;Storage:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time.;