Cat#:RP-3378H;Product Name:Recombinant Human GDNF Protein;Synonym:GDNF; glial cell derived neurotrophic factor; HFB1-GDNF; glial cell line-derived neurotrophic factor; ATF; HSCR3; astrocyte-derived trophic factor；ATF1; ATF2;Description:Recombinant human Glial derived Neurotrophic Factor/GDNF Protein produced in E.coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. The recombinant human GDNF protein is purified by our unique chromatographic techniques.;Source:E.coli;AA Sequence:MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI.;Purity:Greater than 95.0% as determined by RP-HPLC and SDS-PAGE.;Bioactivity:The ED50 of recombinant human GDNF protein was determined by the proliferation of rat C6 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1.0x107 units/mg.;Formulation:Recombinant human GDNF protein was lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4.;Stability:Recombinant human GDNF Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized recombinant Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized recombinant human Glial-derived Neurotrophic Factor / GDNF Protein although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution recombinant human GDNF protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;References:Lin LF, Doherty DH, Lile JD, Bektesh S, Collins F (May 1993). "GDNF: a glial cell line-derived neurotrophic factor for midbrain dopaminergic neurons". Science. 260 (5111): 1130–2. ;