Cat#:RP-3367H;Product Name:Recombinant Human GDF11 Protein;Synonym:Growth Differentiation Factor 11, GDF-11, Bone Morphogenetic Protein 11, BMP11.;Description:GDF11 Protein produced in E.coli is a non-glycosylated homodimer containing 2x109 amino acids and having a total molecular mass of 25.0kDa.?;Source:E.coli;AA Sequence:NLGLDCDEHS SESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGQCEYM FMQKYPHTHL VQQANPRGSA GPCCTPTKMS PINMLYFNDK QQIIYGKIPG MVVDRCGCS ;Purity:Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50 as determined by the ability to inhibit alkaline phosphatase activity in ATDC5 cells, is 0.2-0.3ng/ml, corresponding to a specific activity of 5 x 106units/mg.;Formulation:Lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA).;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized GDF11 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. ;Storage:Lyophilized GDF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;
Greater than 97.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.
Bioactivity:
The ED50 as determined by the ability to inhibit alkaline phosphatase activity in ATDC5 cells, is 0.2-0.3ng/ml, corresponding to a specific activity of 5 x 106units/mg.
Formulation:
Lyophilized from a concentrated (1mg/ml) solution containing 0.1% Trifluoroacetic Acid (TFA).
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
It is recommended to reconstitute the lyophilized GDF11 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Storage:
Lyophilized GDF11 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDF11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.