Cat#:RP-3305H;Product Name:Recombinant Human Fumarase Protein;Synonym:MCL, LRCC, HLRCC, MCUL1, FH, Fumarate hydratase, Fumarase.;Description:Fumarase Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 467 amino acids (44-510) and having a molecular mass of 50.2 kDa. Fumarate Hydratase is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MASQNSFRIE YDTFGELKVP NDKYYGAQTV RSTMNFKIGG VTERMPTPVI KAFGILKRAA AEVNQDYGLD PKIANAIMKA ADEVAEGKLN DHFPLVVWQT GSGTQTNMNV NEVISNRAIE MLGGELGSKI PVHPNDHVNK SQSSNDTFPT AMHIAAAIEV HEVLLPGLQK LHDALDAKSK EFAQIIKIGR THTQDAVPLT LGQEFSGYVQ QVKYAMTRIK AAMPRIYELA AG;Purity:Greater than 95.0% as determined by SDS-PAGE.;Bioactivity:Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37°C.;Formulation:The Fumarase protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;
Fumarase Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 467 amino acids (44-510) and having a molecular mass of 50.2 kDa. Fumarate Hydratase is purified by proprietary chromatographic techniques.
Specific activity is > 25 unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Malate to Fumarate per minute at pH 7.5 at 37°C.
Formulation:
The Fumarase protein solution (1mg/ml) contains 20mM Tris-HCl, pH-8.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.