Cat#:RP-3230H;Product Name:Recombinant Human FGF20 Protein;Synonym:Fibroblast Growth Factor 20, FGF-20, RHDA2, FGF20.;Description:FGF20 Protein (1-211) produced in E.coli is a single, non-glycosylated, polypeptide chain containing 217 amino acids and having a molecular mass of 24kDa. The FGF-20 is fused to a 6 amino acid His tag [HHHHHH] at N-terminus and purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MHHHHHHAPL AEVGGFLGGL EGLGQQVGSH FLLPPAGERP PLLGERRSAA ERSARGGPGA AQLAHLHGIL RRRQLYCRTG FHLQILPDGS VQGTRQDHSL FGILEFISVA VGLVSIRGVD SGLYLGMNDK GELYGSEKLT SECIFREQFE ENWYNTYSSN IYKHGDTGRR YFVALNKDGT PRDGARSKRH QKFTHFLPRP VDPERVPELY KDLLMYT.;Purity:Greater than 97.0% as determined by SDS-PAGE.;Bioactivity:The ED50, as measured in a proliferation assay using mouse NR6R-3T3 cells, is less than 2.5ng/ml.;Formulation:Lyophilized from a 0.2µm filtered solution in MOPS, (NH4)2SO4, DTT and EDTA.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized FGF-20 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized FGF20 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-20 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;