Cat#:RP-3031H;Product Name:Recombinant Human EGF Protein;Synonym:Urogastrone, URG, EGF.;Description:Epidermal Growth Factor Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton. The EGF is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.;Purity:Greater than 98.0% as determined by: (a) Analysis by HPLC. (b) Analysis by SDS-PAGE.;Bioactivity:The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg.;Formulation:The protein was lyophilized from a concentrated (1mg/ml) solution containing PBS pH-7.4.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.;Storage:Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.;