Cat#:RP-2817H;Product Name:Recombinant Human DBR1 Protein;Synonym:Debranching RNA Lariats 1, Lariat Debranching Enzyme, Debranching Enzyme Homolog 1, RNA Lariat Debranching Enzyme, EC 3.1.-.-.;Description:DBR1 Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (228-544) and having a molecular mass of 38kDa (molecular size on SDS-PAGE will appear higher). DBR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGSSHHHHHH SSGLVPRGSH MGSHVKFAAL MQHQAKDKGQ TARATKFLAL DKCLPHRDFL QILEIEHDPS APDYLEYDIE WLTILRATDD LINVTGRLWN MPENNGLHAR WDYSATEEGM KEVLEKLNHD LKVPCNFSVT AACYDPSKPQ TQMQLIHRIN PQTTEFCAQL GIIDINVRLQ KSKEEHHVCG EYEEQDDVES NDSGEDQSEY NTDTSALSSI NPDEIMLDEE ED;Purity:Greater than 90.0% as determined by SDS-PAGE.;Bioactivity:Please contact us for detailed information;Formulation:The DBR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Storage:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.;
DBR1 Protein produced in E.coli is a single, non-glycosylated polypeptide chain containing 340 amino acids (228-544) and having a molecular mass of 38kDa (molecular size on SDS-PAGE will appear higher). DBR1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
The DBR1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.15M NaCl, 1mM DTT and 20% glycerol.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Storage:
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.