Cat#:RP-2752H;Product Name:Recombinant Human CTGF Protein;Synonym:CCN2, NOV2, HCS24, IGFBP8, MGC102839, CTGF, Connective Tissue Growth Factor.;Description:CTGF Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.2 kDa. The CTGF is purified by proprietary chromatographic techniques.;Source:E.coli;AA Sequence:MGKKCIRTPK ISKPIKFELS GCTSMKTYRA KFCGVCTDGR CCTPHRTTTL PVEFKCPDGE VMKKNMMFIK TCACHYNCPG DNDIFESLYY RKMYGDMA.;Purity:Purity of CTGF is Greater than 95% as determined by SDS-PAGE.;Bioactivity:Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1-2µg/ml, corresponding to a specific activity of 500-1000units/mg.;Formulation:CTGF was lyophilized from 1mg/ml solution containing 10mM NaAcetate buffer pH-6.;Stability:Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃;Reconstitution:Reconstitute at 0.1 mg/ml with 5mM NaAcetate, pH-6.;Storage:Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.;
CTGF Protein produced in E.coli is a single, non-glycosylated, polypeptide chain containing 98 amino acids and having a molecular mass of 11.2 kDa. The CTGF is purified by proprietary chromatographic techniques.
Purity of CTGF is Greater than 95% as determined by SDS-PAGE.
Bioactivity:
Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1-2µg/ml, corresponding to a specific activity of 500-1000units/mg.
Formulation:
CTGF was lyophilized from 1mg/ml solution containing 10mM NaAcetate buffer pH-6.
Stability:
Recombinant Proteins are stable for up to 1 year from date of receipt at -70℃
Reconstitution:
Reconstitute at 0.1 mg/ml with 5mM NaAcetate, pH-6.
Storage:
Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.